Honey has very effective wound-healing properties and its use with delicate corneal tissue is a very important discovery. The significance of this study shows Acacia honey, with no preservatives necessary, sped healing of tissue as compared with conventional treatment, which rely on preservatives that also have negative side effects, such as cytotoxicity...
Effect of acacia honey on cultured rabbit corneal keratocytes
BMC Cell Biology 2014, May
Acacia honey is a natural product which has proven to have therapeutic effects on skin wound healing, but its potential healing effects in corneal wound healing have not been studied. This study aimed to explore the effects of Acacia honey (AH) on corneal keratocytes morphology, proliferative capacity, cell cycle, gene and protein analyses. Keratocytes from the corneal stroma of six
white rabbits were isolated and cultured until passage 1. The optimal dose of
AH in the basal medium (FD) and medium containing serum (FDS) for keratocytes
proliferation was identified using MTT assay. The morphological changes, gene
and protein expressions of aldehyde dehydrogenase (ALDH), marker for quiescent
keratocytes and vimentin, marker for fibroblasts were detected using q-RTPCR
and immunocytochemistry respectively. Flowcytometry was performed to evaluate
the cell cycle analysis of corneal keratocytes.
Cultured keratocytes supplemented with AH showed no morphological changes compared to control. Keratocytes cultured in FD and FDS media supplemented with 0.025% AH showed optimal proliferative potential compared with FD and FDS media, respectively.
Gene expressions of ADLH and
vimentin were increased in keratocytes cultured with AH enriched media. All
proteins were expressed in keratocytes cultured in all media in accordance to
the gene expression findings. No chromosomal changes were detected in
keratocytes in AH enriched media.
|Corneal keratocytes in 4 different media after 48hrs|
Corneal keratocytes cultured in media supplemented with 0.025% AH showed an increase in proliferative capacity while retaining their morphology, gene and protein expressions with normal cell cycle. The results of the present study show promising role of AH role in accelerating the initial stage of corneal wound healing.
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