The anti-tumor properties of Propolis continue to be discovered in more diverse geographical locations. Fortunately, its effects are consistently proven very positive and very complementary with other treatments.
Assessing the Anti-tumour Properties of Iraqi Propolis in vitro and in vivo
Food and Chemical Toxicology; 26 January 2012
► We selected one propolis extract with highest cytotoxic effects on different cancer cell lines.
► We have investigated the chemical composition in quantitative manner by using HPLC-ESI-MS.
► We have elucidated the cell cycle perturbations induced by propolis compound M. This is the first study that investigated cell cycle perturbations induced by M using BrdU/DNA assay.
The study was designed to evaluate anti-tumour properties of Iraqi propolis collected from
region (M) on HL-60 and HCT-116 cell lines and on HCT-116 in vivo. Mosul
M induced an inhibitory effect against the proliferation of HL-60 and colony potential of HCT-116 cells. The apoptosis in HL-60 cells was associated with down-regulation of Bcl-2 and activation of Bax, while in HCT-116 cells, necrotic features were observed; size of cells was dramatically increased by swelling of cytoplasm and loss of membrane integrity, cell rupture and release of cellular contents.
Analysis of BrdU/DNA cell cycle in both cell lines showed that M induced cell cycle perturbations in both BrdU positive and BrdU negative cells. The exposure of HL-60 to M caused γ-H2AX in a dose dependent manner and was associated with induction of apoptosis.
The experiments in HCT-116 tumor-bearing mice showed that oral administration of propolis at doses that caused no detectable toxicity was associated with a decrease in mitotic cells and an increase in endoreduplications, increased p53 and decreased Ki-67 expression of cells in tumor sections.
This study provides the rationale to investigate the potential beneficial effect of propolis in the diet of patients receiving anti-cancer therapies.